RT-qPCR Master Mix – SATO

Overview:

The SATO RT-qPCR Kits enable sensitive quantification of RNA targets by real-time one-step PCR using sequence-specific probes. The combination of a Hot-Start DNA Polymerase and a unique PCR buffer system in the ready-to-use master mix ensures highly sensitive qRT-PCR on any real-time thermocycler without the need for optimization.

Product details:

SATO RT-qPCR Master Mix has a unique buffer containing a balanced combination of K⁺ and NH₄⁺ ions that promotes highly specific annealing of primers and probes to the PCR template. Designed for use with all types of sequence-specific probes, this master mix includes hydrolysis probe detection, FRET probes, and Molecular Beacons. The Hot Start DNA Polymerase prevents the formation of nonspecific products. Our RT-qPCR Master Mix also contains dUTP, allowing pre-treatment with uracil-N-glycosylase (UNG) prior to starting PCR, which ensures that any contaminating PCR products do not affect subsequent PCR reactions.

Quality:

All AMD kits manufactured under high quality standardisation methods with unique precision and sensitive technology when compared to the most of famous and approved diagnostic commercial RT-qPCR Master Mix.

Sensitivity:

AMD RT-qPCR Master Mix is a very sensitive kit, detecting a target sequence at over 6 logs of template dilution under our validation methods and devices.

Specificity:

AMD RT-qPCR Probe Master Mix kit is up to 100% specific towards a gene of interest under our validation methods and devices.

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