Colistin antibiotics are considered the last resort drug, treating patients with multi-drug resistant infections caused by gram-negative bacteria. However, with the increased use of colistin antibiotics, caused by the plasmid located Mobile Colistin Resistance (mcr) genes, has lead to the spread of resistance toward these drugs. Three genes of mcr have been strongly associated with Colistin resistance: mcr-1, mcr-2 and mcr-3. It is of great concern that if these variants spread widely, this may cause large outbreaks of untreatable infections.
Mcr genes protects the bacterial cell death by encoding a phosphatidylehtanolamine transferase, which transfers phosphatidylehtanolamine residues to Lipid A on the cell membrane. The presence of this residue reduces the binding affinity of colistin to Lipid A, hence leading inactivity of the colistin antibiotics
The range of assays described below are in vitro PCR reaction assays for the quantitative determination of mcr-1, mcr-2 and mcr-3 genes within bacterial cells. Gene expression can be measured using samples such as whole blood based on Taqman detection method for mcr-1, mcr-2 and mcr-3 genes with a highly sensitive one step qPCR kit.
Quality: All AMD kits are manufactured under high-quality standardization methods with unique precision and sensitive technology when compared to the most of the famous and approved diagnostic commercial Colistin resistance qPCR assays.
Specificity: AMD Colistin resistance kit shows up to 100% specificity for mcr-1, mcr-2 and mcr-3 genes under our validation methods and devices.
When placing an order, please quote the catalog no.
|Product name||Technology||Target Genes||Package||Cat No|
|Colistin resistance||One step Real time PCR||mcr-1, mcr-2 and mcr-3||100 reactions||KD347525-100|