qPCR Master Mix – SATO

Overview:

The SATO qPCR kit enables sensitive quantification of gDNA and cDNA targets using sequence-specific probes. The combination of a “Hot Start” DNA Polymerase and a unique PCR buffer system ensures a highly sensitive qPCR on any real-time thermocycler without the need for optimization

Product details:

Designed for use with all types of sequence-specific probes, the SATO qPCR Master mix includes hydrolysis probe detection, FRET probes, and Molecular Beacons. The SATO qPCR Master Mix contains a unique PCR buffer with a balanced combination of K⁺ and NH₄⁺ ions, promoting specific primer annealing, enabling high PCR specificity and sensitivity. The Hot Start DNA Polymerase prevents the formation of nonspecific products. qPCR Master Mix also contains dUTP, allowing pre-treatment with uracil-N-glycosylase (UNG) prior to starting PCR, which ensures that any contaminating PCR products do not affect subsequent PCR reactions.

Quality:

All AMD kits manufactured under high quality standardization methods with unique precision and sensitive technology when compared by the most of famous and approved diagnostic commercial qPCR Master Mix.

Sensitivity:

AMD qPCR Master Mix is very sensitive kit, with an efficiency of 97% over 6 logs of template dilution under our validation methods and devices. As little as 1 pg of IL8 transcript was sensitively detected.

Specificity:

AMD qPCR Master Mix kit is up to 100% specific for target transcripts under our validation methods and devices.