β-lactam antibiotics are commonly used medication to treat bacterial infections, however certain species including Escherichia coli and Klebsiella pneumoniae, have acquired resistance through expressing β-lactamase. β-lactamase hydrolyses β-lactam rendering ineffective against populations expressing the enzyme. There are a number of genes expressing β-lactamase, many of which are located in plasmids, which cause specific resistance to certain types of antibiotics. Extended Spectrum β-lactamase cause resistance to a range of different antibiotics, hence why prognosis is poor, if patients have ESBL infections. TEM, SHV and GES are genes strongly associated with ESBL resistance. These resistant genes are plasmid mediated β-lactamases, commonly found in gram-negative bacteria e.g. Haemophilus influenzae, Klebsiella pneumoniae and Neisseria gonorrhoeae. They are effective against penicillins and certain cephalosporins.
The range of assays described below are in vitro PCR reaction assays for the quantitative determination of TEM, SHV and GES genes within bacterial cells. Gene expression can be measured using samples such as whole blood based on Taqman detection method for TEM, SHV and GES genes with a highly sensitive one step qPCR kit.
Quality: All AMD kits are manufactured under high-quality standardization methods with unique precision and sensitive technology when compared to the most of the famous and approved diagnostic commercial Extended spectrum β-lactamase qPCR assays.
Specificity: AMD Extended spectrum β-lactamase kit shows up to 100% specificity for TEM, SHV and GES genes under our validation methods and devices.
When placing an order, please quote the reference number:
|Product name||Technology||Target Genes||Package||Cat No|
|AMD Extended spectrum β-lactamase kit||One step Real time PCR||TEM, SHV and GES||100 reactions||KD347521-100|