Overview.

Tetracycline is a broad-spectrum antibiotic showing effectiveness against gram positive and gram negative bacteria, mycoplasmas, and protozoan parasites. The antibiotic prevents the production of protein synthesis through its ability to stop the attachment of aminoacyl-tRNA to the ribosomal acceptor site. However, due to its high effectiveness and inexpensive production, Tetracycline resistance is steadily on the rise. Genes like tetA and tetB cause the removal of tetracycline from the organism, preventing any protein synthesis inhibition from taking place.

Vancomycin has been the preferred antibiotic when treating infections caused by gram-positive infections and resistant to methicillin. However, resistance towards vancomycin is on the rise, but developed in the laboratories rather than due to intense prescribing of the antibiotic. Organisms like  E. faecalis and E. faecium express vancomycin resistant genes e.g. vanB.

The AMD Tetracycline and Vancomycin resistance kit allow for quick quantitative identification of organisms expressing these specific antibiotic resistant genes.

Product Details:

The range of assays described below are in vitro PCR reaction assays for the quantitative determination of tetA, tetB and vanB genes within antibiotic resistant bacterial cells. Gene expression can be measured using samples such as whole blood based on Taqman detection method for tetA, tetB and vanB with a highly sensitive one step qPCR kit.

Quality: All AMD kits are manufactured under high-quality standardization methods with unique precision and sensitive technology when compared to the most of the famous and approved diagnostic commercial Tetracycline, Vancomycin and Erythromycin qPCR assays.

Specificity: AMD Tetracycline, vancomycin and erythromycin resistance kit shows up to 100% specificity for tetA, tetB and vanB genes under our validation methods and devices.

ORDER INFORMATION

When placing an order, please quote the reference number:

Product name	Technology	Target Genes	Package	Cat No
Tetracycline and Vancomycin resistance kit	One step Real time PCR	tetA, tetB and vanB	100 reactions	KD347523-100