β-lactam antibiotics are commonly used medication to treat bacterial infections, however certain species including Escherichia coli and Klebsiella pneumoniae, have acquired resistance through expressing β-lactamase. β-lactamase hydrolyses β-lactam rendering ineffective against populations expressing the enzyme. There are a number of genes expressing β-lactamase, many of which are located in plasmids, which cause specific resistance to certain types of antibiotics. Some OXA genes encode carbapenemase (a type of β-lactamase) and were among the earliest to be detected as antibiotic resistant genes. Carbapenemases hydrolyze carbapenem based antibiotics e.g. Ertapenem and Doripenem, rendering these broad spectrum drugs useless.
The range of assays described below are in vitro PCR reaction assays for the quantitative determination of OXA23, OXA48 and OX58 genes within bacterial cells. Gene expression can be measured using samples such as whole blood based on Taqman detection method for OXA23, OXA48 and OX58 genes with a highly sensitive one step qPCR kit.
Quality: All AMD kits are manufactured under high-quality standardization methods with unique precision and sensitive technology when compared to the most of the famous and approved diagnostic commercial OXA β-lactamase qPCR assays.
Specificity: AMD OXA β-lactamase kit shows up to 100% specificity for OXA23, OXA48 and OX58 genes under our validation methods and devices.
When placing an order, please quote the reference number:
|Product name||Technology||Target Genes||Package||Cat No|
|OXA β-lactamase kit||One step Real time PCR||OXA23, OXA48 and OX58||100 reactions||KD347522-100|