Zena Max – COVID-19

Zena Max – AMD SARS-COV-2 Real Time PCR Detection Kit CE-IVD

Overview.

Coronavirus disease 2019 (COVID 19)  is caused by a severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) that was first detected in Wuhan City, Hubei Province, China. The virus is contagious and can be spread from human to human primarily through the exchange of mucus droplets that are expelled through sneezes or coughs. The SARS-CoV-2 (2019-nCoV)  is a severe acute respiratory syndrome coronavirus outbreak is an important reminder that the global community must strengthen national and international programs for detection and response to future disease outbreaks.

Product Details:

This kit detects the presence of COVID-19 using one-step RT-qPCR (both reactions in the same tube) by first reverse transcribing the genomic RNA target to cDNA, followed by amplification of the assay target and detection by the hydrolysis probe method of qPCR. The assay consists of a forward primer, a reverse primer and a probe labelled with the 5’ FAM™ and ROX reporter dye with a 3’ quencher. As the new target cDNA strand is synthesised, the tightly bound probe is cleaved by the 5’ to 3’ exonuclease activity of Taq polymerase which releases the fluorescent reporter from the quencher and substantially increases the fluorescent signal. The point at which the fluorescence becomes detectable above the background, the quantification cycle (Cq), is proportional to the amount of target present in the sample. The primers and probe sets target the RdRp gene and N Gene which had previously been used in the identification of the SARS coronavirus, however, there is no cross-reactivity with this or any other coronavirus sequenced thus far. .These assays are both incorporated into a ready-to-use PCR master mix which utilises hot start technology, thus minimising non-specific reactions and ensuring maximum sensitivity.

This in Vitro diagnostic kit provides qualitative detection.

Quality: All AMD kits are manufactured under high quality standard methods and unique precision, comparable with other leading commercial SARS-CoV-2 virus diagnostic kits.. 

Sensitivity: AMD SARS-CoV-2 Virus RNA kit is very sensitive kit reaches up to one copy “rxn volume 25ul” under our validation methods and devices.

Specificity: AMD Human SARS-CoV-2 Virus RNA is very specific up to 100% for Human SARS-CoV-2 Virus RNA under our validation methods and devices.

ORDER INFORMATION

Product name Technology Package Catalogue  No
AMD Human SARS-CoV-2 detection kit One step Real time PCR 100 reactions KD145904-100